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Catalog Number | orb1530090 |
---|---|
Category | Antibodies |
Description | AMPK alpha 1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | between 350 and 400 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | AMPK alpha 1 |
UniProt ID | Q13131 |
NCBI | BAA36547.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | AMP -activate kinase alpha 1 subunit; AMPK subunit Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human AMPK alpha1 by western blot.Samples: Whole cell lysate from A-549, Jurkat, U2OS, HEK293T, and HeLa cells. Antibody: Affinity purified rabbit anti-AMPK alpha1 antibody at 0.04 µg/ml.
Detection of human AMPK alpha 1 by immunohistochemistry. Sample: FFPE section of human skeletal muscle. Antibody: Affinity purified rabbit anti-AMPK alpha 1 used at a dilution of 1:1, 000 (1µg/ml).
Detection of human AMPK alpha1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-AMPK alpha1 antibody.
FC, IHC-P | |
Bovine, Canine, Equine, Gallus, Porcine | |
Human, Mouse, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Human, Monkey, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IHC-P | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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