Cart summary

You have no items in your shopping cart.

AMBRA1 Antibody: Biotin

Catalog Number: orb384099

DispatchUsually dispatched within 2-3 weeks
$ 590.00
Catalog Numberorb384099
CategoryAntibodies
DescriptionRabbit polyclonal antibody against AMBRA1 conjugated to Biotin.
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, ICC, IF, IHC, WB
ReactivityHuman, Rat
ImmunogenSynthetic peptide of Human AMBRA1 (aa. 200-300)
Concentration1 mg/ml
Dilution rangeWB (1:1000), ICC/IF (1:100)
ConjugationBiotin
MW142 kDa
TargetAMBRA1
Entrez55626
UniProt IDQ9C0C7
NCBINP_001254711.1
StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
Alternative namesAMBRA1 antibody, Activating molecule in BECN1-regu
Read more...
NoteFor research use only
Application notesA 1:1000 dilution of SPC-644 was sufficient for detection of AMBRA1 in 15 µg of Rat Brain Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Expiration Date12 months from date of receipt.
AMBRA1 Antibody: Biotin

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AMBRA1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMBRA1 Antibody (D) Composite.

AMBRA1 Antibody: Biotin

Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band (s): 50 kDa.

  • AMBRA1 Rabbit Polyclonal Antibody (Biotin) [orb458314]

    IF,  IHC-Fr,  IHC-P

    Canine, Equine, Gallus, Human, Mouse, Rabbit

    Rat

    Rabbit

    Polyclonal

    Biotin

    100 μl