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Catalog Number | orb384099 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against AMBRA1 conjugated to Biotin. |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IF, IHC, WB |
Reactivity | Human, Rat |
Immunogen | Synthetic peptide of Human AMBRA1 (aa. 200-300) |
Concentration | 1 mg/ml |
Dilution range | WB (1:1000), ICC/IF (1:100) |
Conjugation | Biotin |
MW | 142 kDa |
Target | AMBRA1 |
Entrez | 55626 |
UniProt ID | Q9C0C7 |
NCBI | NP_001254711.1 |
Storage | Conjugated antibodies should be stored according to the product label |
Buffer/Preservatives | 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS |
Alternative names | AMBRA1 antibody, Activating molecule in BECN1-regu Read more... |
Note | For research use only |
Application notes | A 1:1000 dilution of SPC-644 was sufficient for detection of AMBRA1 in 15 µg of Rat Brain Lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody. |
Expiration Date | 12 months from date of receipt. |
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-AMBRA1 Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) AMBRA1 Antibody (D) Composite.
Western blot analysis of Rat Brain cell lysates showing detection of 142.5 kDa AMBRA1 protein using Rabbit Anti-AMBRA1 Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain cell lysates. Load: 15 μg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-AMBRA1 Polyclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 142.5 kDa. Other Band (s): 50 kDa.
IF, IHC-Fr, IHC-P | |
Canine, Equine, Gallus, Human, Mouse, Rabbit | |
Rat | |
Rabbit | |
Polyclonal | |
Biotin |