AMACR Antibody (C-term)

• Catalog Number: orb1939162
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18736
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 42387 Da
• Target: This AMACR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the C-terminal region of human AMACR.
• UniProt ID: Q9UHK6
• NCBI: NP_001161067.1, NP_055139.4, NP_976316.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Alpha-methylacyl-CoA racemase, 2-methylacyl-CoA ra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-AMACR Antibody (C-term) at 1:1000 dilution. Lane 1: human kidney lysate. Lane 2: LNCaP whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 42 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

AMACR Antibody (C-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AMACR antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AMACR antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

AMACR Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35 ug/lane). This demonstrates the AMACR antibody detected the AMACR protein (arrow).

Confocal immunofluorescent analysis of AMACR Antibody (C-term) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).