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Catalog Number | orb1252552 |
---|---|
Category | Antibodies |
Description | ALPP Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ALPP/870 |
Tested applications | FC, IF, IHC-P |
Reactivity | Human |
Isotype | IgG2b, kappa |
Immunogen | Recombinant full-length human ALPP protein was used as the immunogen for the placental Alkaline Phosphatase antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | ALPP |
UniProt ID | P05187 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Alkaline phosphatase, placental type, Alkaline pho Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the placental Alkaline Phosphatase antibody should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human placenta stained with placental Alkaline Phosphatase antibody (ALPP/870).
FC, ICC, IF, IHC, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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