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Catalog Number | orb389385 |
---|---|
Category | Antibodies |
Description | Placental alkaline phosphatase (PLAP)/ALPP Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, ICC, IF, IHC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ALPP (403-433aa KARDRKAYTVLLYGNGPGYVLKDGARPDVTE). |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 57954 MW |
UniProt ID | P05187 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Alkaline phosphatase, placental type;3.1.3.1;Alkal Read more... |
Note | For research use only |
Application notes | Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
Flow Cytometry analysis of SiHa cells using anti-ALPP antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.
WB analysis of ALPP using anti-ALPP antibody.Lane 1:human placenta tissue; 2:human placenta tissue.
IF analysis of ALPP using anti-ALPP antibody. ALPP was detected in a paraffin-embedded section of human placenta tissue.
IF analysis of ALPP using anti- ALPP antibody. ALPP was detected in immunocytochemical section of HELA cells.
IHC analysis of ALPP using anti-ALPP antibody. ALPP was detected in a paraffin-embedded section of human placenta tissue.
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