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Catalog Number | orb1714208 |
---|---|
Category | Proteins |
Description | Mouse Recombinant Alpha Synuclein Pre-formed Fibrils (Type 1) |
Tested applications | In vitro, In vivo, SDS-PAGE, WB |
Reactivity | Mouse |
Tag | No tag |
Concentration | Lot/batch specific. See included datasheet. |
Purity | > 95% |
MW | ~14.46 kDa |
Target | Alpha Synuclein |
UniProt ID | O55042 |
Protein Sequence | MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA |
Protein Length | Full Length |
Source | Recombinant |
Expression System | E. coli |
Biological Activity | Endogenous alpha-synuclein phosphorylation. 100 µM alpha synuclein protein monomer (SPR-323) seeded with 10 uM alpha synuclein protein PFF (SPR-324) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated an increased fluorescence intensity after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader. |
Storage | -80°C |
Buffer/Preservatives | PBS pH 7.4 |
Alternative names | Alpha synuclein PFFs, Alpha synuclein PFF, Alpha s Read more... |
Note | For research use only |
Application notes | Certified > 95% pure using SDS-PAGE analysis. Low endotoxin < 5 EU/mL @ 2mg/mL. |
Expiration Date | 6 months from date of receipt. |
In vitro, In vivo, SDS-PAGE, WB | |
> 95% | |
~14.46 kDa | |
Recombinant |
In vitro, In vivo, SDS-PAGE, WB | |
> 95% | |
~14.46 kDa | |
Recombinant |
In vitro, In vivo, SDS-PAGE, WB | |
> 95% | |
~14.46 kDa | |
Recombinant |
In vitro, In vivo, SDS-PAGE, WB | |
> 95% | |
~14.46 kDa | |
Recombinant |
In vitro, In vivo, SDS-PAGE, WB | |
> 95% | |
~14.46 kDa | |
Recombinant |
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