Alpha Synuclein Pre-formed Fibrils (Type 1)

• Catalog Number: orb1714207
• Category: Proteins
• Description: Mouse Recombinant Alpha Synuclein Pre-formed Fibrils (Type 1)
• Tested applications: In vitro, In vivo, SDS-PAGE, WB
• Reactivity: Mouse
• Tag: No tag
• Concentration: Lot/batch specific. See included datasheet.
• Purity: > 95%
• MW: ~14.46 kDa
• Target: Alpha Synuclein
• UniProt ID: O55042
• Protein Sequence: MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGNIAA ATGFVKKDQM GKGEEGYPQE GILEDMPVDP GSEAYEMPSE EGYQDYEPEA
• Protein Length: Full Length
• Source: Recombinant
• Expression System: E. coli
• Biological Activity: Endogenous alpha-synuclein phosphorylation. 100 µM alpha synuclein protein monomer (SPR-323) seeded with 10 uM alpha synuclein protein PFF (SPR-324) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated an increased fluorescence intensity after incubation at 37°C with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
• Storage: -80°C
• Buffer/Preservatives: PBS pH 7.4
• Alternative names: Alpha synuclein PFFs, Alpha synuclein PFF, Alpha s
• Note: For research use only
• Application notes: Certified > 95% pure using SDS-PAGE analysis. Low endotoxin < 5 EU/mL @ 2mg/mL.
• Expiration Date: 6 months from date of receipt.