Alpha Synuclein Antibody

SKU: orb536144

Description

Rabbit polyclonal antibody to alpha Synuclein

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SPC-800 was sufficient for detection of Alpha Synuclein in 15 μg of mouse Brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Human alpha Synuclein Fibril
• Target: Alpha Synuclein
• Molecular Weight: 14.46kDa
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Alpha Synuclein, α-Synuclein, SNCA, Snca, SYN, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Parkinson disease familial 1, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease (autosomal dominant, Lewy body) 4

Immunohistochemistry analysis using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Tissue: Cerebellum. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody.

Western blot analysis of Mouse Brain showing detection of ~14.46kDa Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~14.46kDa. Other Band (s): Higher molecular weight oligomers.

Western blot analysis of Human Recombinant Protein showing detection of Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: MW ladder. Lane 2: Alpha Synuclein. Lane 3: Beta Synuclein. Lane 4: Gamma Synuclein. Load: 0.5 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Alpha synuclein antibody shows cross-reactivity with beta synuclein but not gamma synuclein.

Western blot analysis of Human Truncated Alpha Synuclein Protein showing detection of Alpha Synuclein protein using Rabbit Anti-Alpha Synuclein Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: hASYN aa 1-114 (5 uL). Load: 5 uL. Block: 5% Skim Milk Powder in TBST. Primary Antibody: Rabbit Anti-Alpha Synuclein Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.

Quick Database Links

Gene Symbol

Alpha Synuclein

UniProt

P37840

NCBI Gene ID

6622

RefSeq (Protein):NP_000336.1