Alpha Amylase Antibody

SKU: orb344567

Description

Alpha Amylase antibody

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Bacteria
• Application Notes:
  Anti-Alpha Amylase has been tested by ELISA and western blot and is assayed against 1.0 ug of a-Amylase [Bacillus amyloliquefaciens] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: a-Amylase [Bacillus amyloliquefaciens]
• Purity: Alpha Amylase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-Amylase [Bacillus amyloliquefaciens]. Cross reactivity against a-Amylase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Alpha Amylase Antibody, Alpha-amylase, 1,4-alpha-D-glucan glucanohydrolase

Quick Database Links

UniProt

P00692

RefSeq (Protein):WP_013352208.1