Alpha-actin-1 antibody

• Catalog Number: orb1473825
• Category: Antibodies
• Description: Mouse monoclonal antibody to Alpha-actin-1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, IH, WB
• Reactivity: Human
• Immunogen: Recombinant fusion protein of human Alpha-actin-1. The exact sequence is proprietary.
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), FC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ACTA1
• Entrez: 58
• UniProt ID: P68133
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: ACTA; Actin alpha skeletal muscle; Alpha-actin-1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Alpha-actin-1 expression in MDAMB231 (A), MDAMB453 (B), Hela (C), mouse liver (D), CEM (E) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 42 kD)

Immunohistochemical analysis of Alpha-actin-1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of A549 cells using Anti-Alpha-actin-1 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.