ACTA1 Antibody

• Catalog Number: orb1252673
• Category: Antibodies
• Description: ACTA1 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HHF35
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit, Rat
• Isotype: IgG1, kappa
• Immunogen: SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ACTA1
• UniProt ID: P68133
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Actin, alpha skeletal muscle, Alpha-actin-1, ACTA1
• Note: For research use only
• Application notes: Western blot: 0.5-1 ug/mlFlow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 ug/mlIHC (FFPE): 0.5-1.0 ug/ml for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC staining of human leiomyosarcoma (10X) with Muscle actin antibody (HHF35).

IHC staining of human leiomyosarcoma (20X) with Muscle actin antibody (HHF35).

IHC testing of FFPE rhabdomyosarcoma with Muscle Actin antibody.

Western blot testing of 1) human muscle and 2) mouse muscle cell lysate with Muscle Actin antibody (clone HHF35).

SDS-PAGE Analysis of Purified, BSA-Free Muscle Actin Antibody (clone HHF35). Confirmation of Integrity and Purity of the Antibody.