Alpha 1 Fetoprotein antibody

• Catalog Number: orb43645
• Category: Antibodies
• Description: Mouse monoclonal antibody to Alpha 1 Fetoprotein
• Clonality: Monoclonal
• Clone Number: AFP-11
• Tested applications: ELISA, IHC-P, RIA, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: purified alpha-Fetoprotein
• Concentration: 1 mg/ml
• Dilution range: ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100). Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT, detection DAB.Western blotting: Recommended dilution: 2 μg/ml, non-reducing conditions.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: alpha-Fetoprotein
• Entrez: 174
• UniProt ID: P02771
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti AFP antibody, anti Alpha fetoglobulin antibod
• Note: For research use only
• Application notes: ELISA: The antibody AFP-11 has been tested as the capture antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-01 (cat. no. 11-259-C100). Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: hepatocellular carcinoma; heat mediated antigen retrieval (sodium citrate buffer); mAb incubation 1 hour / RT, detection DAB.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry staining of hepatocellular carcinoma (fetal liver; paraffin-embedded sections) with anti-human alpha-Fetoprotein (AFP-11).

Western blotting analysis of human alpha-fetoprotein using mouse monoclonal antibody AFP-11 on lysates of HepG2 cell line and Jurkat cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-alpha-fetoprotein followed by IRDye800-conjugated anti-mouse secondary antibody. Alpha-fetoprotein was detected at approximately 60 kDa.