AFP Antibody

SKU: orb1239167

Description

AFP Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine, Sheep
• Application Notes:
  AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: AFP antibody was raised against a 14 amino acid synthetic peptide near the center of human AFP.The immunogen is located within amino acids 340 - 390 of AFP.
• Target: AFP
• Purification: AFP Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: AFP Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein

Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 µg/mL and (B) 2 µg/mL.

Immunohistochemistry of AFP in mouse liver tissue with AFP antibody at 2.5 µg/ml.

Immunofluorescence of AFP in rat liver tissue with AFP antibody at 20 µg/mL.

Immunohistochemistry of AFP in rat liver tissue with AFP antibody at 10 µg/mL.

Immunofluorescence of AFP in mouse liver tissue with AFP antibody at 20 µg/mL. Red: AFP Antibody (orb1239167) Blue: DAPI staining.

Quick Database Links

Gene Symbol

AFP

UniProt

P02771

RefSeq (Protein):NP_001125