AFP Antibody

• Catalog Number: orb1239167
• Category: Antibodies
• Description: AFP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: AFP antibody was raised against a 14 amino acid synthetic peptide near the center of human AFP.The immunogen is located within amino acids 340 - 390 of AFP.
• Concentration: 1 mg/mL
• Dilution range: AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: AFP
• UniProt ID: P02771
• NCBI: NP_001125
• Storage: AFP antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: AFP Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: AFP Antibody: FETA, HPAFP, Alpha-fetoprotein, Alph
• Note: For research use only
• Application notes: AFP antibody can be used for detection of AFP by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AFP in fetal human liver tissue lysate with AFP antibody at (A) 1 µg/mL and (B) 2 µg/mL.

Immunohistochemistry of AFP in mouse liver tissue with AFP antibody at 2.5 µg/ml.

Immunofluorescence of AFP in rat liver tissue with AFP antibody at 20 µg/mL.

Immunohistochemistry of AFP in rat liver tissue with AFP antibody at 10 µg/mL.

Immunofluorescence of AFP in mouse liver tissue with AFP antibody at 20 µg/mL. Red: AFP Antibody (orb1239167) Blue: DAPI staining.