Alpha 1 Fetoprotein antibody

• Catalog Number: orb43644
• Category: Antibodies
• Description: Mouse monoclonal antibody to Alpha 1 Fetoprotein
• Clonality: Monoclonal
• Clone Number: AFP-01
• Tested applications: ELISA, ICC, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: purified alpha-Fetoprotein
• Concentration: 1 mg/ml
• Dilution range: ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100).Western blotting: Recommended dilution: 1-2 μg/ml; non-reducing conditions.Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• Target: alpha-Fetoprotein
• Entrez: 174
• UniProt ID: P02771
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti AFP antibody, anti Alpha fetoglobulin antibod
• Note: For research use only
• Application notes: ELISA: The antibody AFP-01 has been tested as the detection antibody in a sandwich ELISA for analysis of human alpha-fetoprotein in combination with antibody AFP-11 (cat. no. 11-384-C100). Western blotting: Positive material: Hep G2 human hepatocellular carcinoma cell line; both non-reducing and reducing conditions. Immunoprecipitation: Interaction of the antibody AFP-01 with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human alpha-fetoprotein using mouse monoclonal antibody AFP-01 on lysates of HepG2 cell line and Jurkat cell line (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-alpha-fetoprotein followed by IRDye800-conjugated anti-mouse secondary antibody. Alpha-fetoprotein was detected at approximately 60 kDa.