Alpha-1-Acid Glycoprotein antibody (Biotin)

• Catalog Number: orb344748
• Category: Antibodies
• Description: Alpha-1-Acid Glycoprotein antibody (Biotin)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Acid Glycoprotein Antibody was produced by repeated immunizations with a-1-Acid Glycoprotein isolated from human plasma.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Alpha-1-Acid Glycoprotein Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-1-Acid Glycoprotein [Human Plasma]. Cross reactivity against a-1-Acid Glycoprotein from other sources may occur but has not been specifically determined.
• Conjugation: Biotin
• UniProt ID: P02763
• NCBI: NP_000598.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Alpha-1-Acid Glycoprotein Antibody bio
• Note: For research use only
• Application notes: Anti-Alpha-1-Acid Glycoprotein Biotin Antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of a-1-Acid Glycoprotein in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.