Alpha-1-Acid Glycoprotein Antibody

SKU: orb344569

Description

Alpha-1-Acid Glycoprotein antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:135,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Alpha-1-Acid Glycoprotein Antibody has been tested by western blot and is suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Alpha-1-Acid Glycoprotein Antibody was produced by repeated immunizations with human plasma a-1-Acid Glycoprotein.
• Purity: Alpha-1-Acid Glycoprotein Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-1-Acid Glycoprotein (Human Plasma). Cross-reactivity against a-1-Acid Glycoprotein from other tissues and species may occur but has not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Alpha-1-Acid Glycoprotein Antibody, AGP1, AGP 1, OMD 1, OMD1, Orosomucoid-1, agpa antibody, Alpha 1 agp antibody, Glycoprotein alpha acid of serum antibody

Western Blot of Alpha-1-Acid Glycoprotein (Human Plasma) Antibody. Lane 1: Alpha-1-Acid Glycoprotein (Human Plasma). Load: 50 ng. Primary antibody: Alpha-1-Acid Glycoprotein (Human Plasma) antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: Predicted at 24 kDa/Observed - 40 kDa; protein migrates at 40 kDa.

Quick Database Links

UniProt

P02763

RefSeq (Protein):NP_000598.2