Alpha-1-Acid Glycoprotein Antibody

SKU: orb344568

Description

Alpha-1-Acid Glycoprotein antibody

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, WB
Dilution range	ELISA: 1:135,000, WB: 1:2,000 - 1:10,000
Reactivity	Human
Application Notes	Anti-Alpha-1-Acid Glycoprotein Antibody has been tested by western blot and is suitable for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Anti-Alpha-1-Acid Glycoprotein Antibody was produced by repeated immunizations with human plasma a-1-Acid Glycoprotein.
Purity	Alpha-1-Acid Glycoprotein Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified a-1-Acid Glycoprotein (Human Plasma). Cross-reactivity against a-1-Acid Glycoprotein from other tissues and species may occur but has not been specifically determined.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.01% (w/v) Sodium Azide
Concentration	1.0 mg/ml
Disclaimer	For research use only

Alternative Names

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rabbit anti-Alpha-1-Acid Glycoprotein Antibody, AGP1, AGP 1, OMD 1, OMD1, Orosomucoid-1, agpa antibody, Alpha 1 agp antibody, Glycoprotein alpha acid of serum antibody

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Western Blot of Alpha-1-Acid Glycoprotein (Human Plasma) Antibody. Lane 1: Alpha-1-Acid Glycoprotein (Human Plasma). Load: 50 ng. Primary antibody: Alpha-1-Acid Glycoprotein (Human Plasma) antibody at 1:1000 overnight at 4°C. Secondary antibody: HRP conjugated rabbit secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: Predicted at 24 kDa/Observed - 40 kDa; protein migrates at 40 kDa.

Quick Database Links

UniProt

P02763

RefSeq (Protein):NP_000598.2

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_000598.2

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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