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ALOX15 Rabbit Polyclonal Antibody

SKU: orb101113

Description

ALOX15 Rabbit Polyclonal Antibody

Research Area

Metabolism Research

Images & Validation

Tested ApplicationsWB
Dilution RangeWB=1:500-2000
ReactivityRat
Predicted ReactivityBovine, Human, Mouse, Porcine

Related Conjugates & Formulations

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from mouse ALOX15/15 Lipoxygenase 1 (581-662/662aa)
TargetALOX15
Molecular Weight73 kDa
PurificationAffinity purified by Protein A
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration1mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

12-LOX; 15-LOX; 15-LOX-1; LOG15; Alox12; Alox12l; LOX15_HUMAN; ALOX15; 12/15-lipoxygenase; Arachidonate 12-lipoxygenase, leukocyte-type (12-LOX); Arachidonate 15-lipoxygenase (15-LOX | 15-LOX-1); Arachidonate omega-6 lipoxygenase; Hepoxilin A3 synthase Alox15; Linoleate 13S-lipoxygenase; LOX15_RAT; Arachidonate 15-lipoxygenase (15-LOX);

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ALOX15 Rabbit Polyclonal Antibody

Tissue/cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37℃ for 20 min, Incubation: Anti-15-LOX Polyclonal Antibody, Unconjugated (orb101113) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

ALOX15 Rabbit Polyclonal Antibody (orb101113)

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Available Sizes

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50 μl
$ 250.00
100 μl
$ 390.00
200 μl
$ 470.00
DispatchUsually dispatched within 5-10 working days
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