ALOX15 Antibody

SKU: orb1246616

Description

ALOX15 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-HYKTDVAVKDDPE
• Target: ALOX15
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2

orb1246616 (0.2 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246616 (2 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246616 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ALOX15

UniProt

P16050

RefSeq (Protein):NP_001131.3