ALOX15 Antibody

• Catalog Number: orb1246616
• Category: Antibodies
• Description: ALOX15 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HYKTDVAVKDDPE
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ALOX15
• UniProt ID: P16050
• NCBI: NP_001131.3
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration: 2ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246616 (0.2 ug/ml) staining of nuclear HeLa lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246616 (2 ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246616 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.