ALK Rabbit Polyclonal Antibody

SKU: orb10073

Description

ALK Rabbit Polyclonal Antibody

Research Area

Cancer Research, Cell Biology, Kinases, Neuroscience, Tumor Biology, Tyrosine Kinase Signaling Pathway

Images & Validation

• Tested Applications: FC
• Dilution Range: Flow-Cyt=1μg /test
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CD246 (329-342/1620aa)
• Target: ALK
• Molecular Weight: 174 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALK_HUMAN; ALK; Anaplastic lymphoma kinase; 2.7.10.1; ALK_MOUSE;

Blank control: Jurkat cells (blue). Primary Antibody: Rabbit Anti-ALK antibody (orb10073), Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions), Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol, The cells were fixed with 2% paraformaldehyde (10 min). Primary antibody (orb10073, 1 µg/1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20000 events was performed.

Quick Database Links

Gene Symbol

ALK

UniProt

Q9UM73