ALIX/PDCD6IP Antibody

• Catalog Number: orb334502
• Category: Antibodies
• Description: ALIX/PDCD6IP Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human ALIX recombinant protein (Position: A2-D330). Human ALIX shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat ALIX, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 96023 MW
• UniProt ID: Q8WUM4
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Programmed cell death 6-interacting protein;PDCD6-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of ALIX using anti-ALIX antibody.Lane 1:human HeLa cell;2:human HepG2 cell;3:human PANC-1 cell;4:rat liver tissue;5:rat RH35 cell;6:mouse liver tissue;7:mouse NIH/3T3 cell.

IF analysis of ALIX using anti-ALIX antibody. ALIX was detected in an immunocytochemical section of T-47D cells.

IHC analysis of ALIX using anti-ALIX antibody.ALIX was detected in paraffin-embedded section of rat liver tissues.

IHC analysis of ALIX using anti-ALIX antibody.ALIX was detected in paraffin-embedded section of huamn lung tissues.

IHC analysis of ALIX using anti-ALIX antibody.ALIX was detected in paraffin-embedded section of huamn oesophagus squama tissues.

IHC analysis of ALIX using anti-ALIX antibody.ALIX was detected in paraffin-embedded section of huamn placenta tissues.

IHC analysis of ALIX using anti-ALIX antibody.ALIX was detected in paraffin-embedded section of mouse intestine tissues.