ALIX/PDCD6IP Antibody(monoclonal, 14D10)

• Catalog Number: orb614091
• Category: Antibodies
• Description: ALIX/PDCD6IP Antibody(monoclonal, 14D10)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 14D10
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: E.coli-derived human PDCD6IP recombinant protein (Position: A2-D330). Human PDCD6IP shares 96.7% and 95.2% amino acid (aa) sequence identity with mouse and rat PDCD6IP, respectively.
• Concentration: 0
• Dilution range: "Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106, Human"
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 96-100 kDa
• UniProt ID: Q8WUM4
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Programmed cell death 6-interacting protein; PDCD6
• Note: For research use only
• Application notes: "Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users." . Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis using anti-PDCD6IP antibody.Lane 1:HeLa cell, Lane 2:HepG2 cell, Lane 3:Jurkat cell, Lane 4:PANC-1 cell, Lane 5:K562 cell, Lane 6:SW579 cell, Lane 7:rat RH35 cell, Lane 8:mouse NIH3T3 cell

IHC analysis of PDCD6IP using anti-PDCD6IP antibody.

IHC analysis of PDCD6IP using anti-PDCD6IP antibody.

IHC analysis of PDCD6IP using anti-PDCD6IP antibody.

IHC analysis of PDCD6IP using anti-PDCD6IP antibody.

IHC analysis of PDCD6IP using anti-PDCD6IP antibody.

IHC analysis of PDCD6IP using anti-PDCD6IP antibody.

IF analysis of PDCD6IP using anti-PDCD6IP antibody.

Flow Cytometry analysis of A431 cells using anti-PDCD6IPantibody(Blue line).Isotype control antibody (Green line) was mouse IgG.Unlabelled sample (Red line) was also used as a control.