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ALDOB Rabbit Polyclonal Antibody
SKU: orb101228
Featured
Description
Research Area
Cell Biology, Energy Metabolism, Metabolism
Images & Validation
−Item 1 of 3
| Tested Applications | ICC, WB |
|---|---|
| Dilution Range | WB=1:500-2000, ICC/IF=1:100-500 |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Mouse, Porcine, Rabbit, Rat, Sheep |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ALDOB (261-364/364aa) |
| Target | ALDOB |
| Molecular Weight | 39 kDa |
| Purification | Affinity purified by Protein A |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ALDB; ALDO B; ALDO2; ALDOB; ALDOB_HUMAN; Aldolase 2; Aldolase B; Aldolase B fructose bisphosphate; Aldolase2; AldolaseB; EC 4.1.2.13; Fructose bisphosphate aldolase B; Fructose-bisphosphate aldolase B; Liver type aldolase; Liver-type aldolase; MS1077.
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HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ALDOB) polyclonal Antibody, Unconjugated (orb101228) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.
Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-ALDOB (orb101228) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.
Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-ALDOB (orb101228) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kDa, Observed band size: 39 kDa.
Quick Database Links
Gene Symbol
ALDOB
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
ALDOB Rabbit Polyclonal Antibody (orb101228)
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