ALDOB Rabbit Polyclonal Antibody

• Catalog Number: orb101228
• Category: Antibodies
• Description: ALDOB Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, WB
• Predicted Reactivity: Bovine, Mouse, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ALDOB (261-364/364aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, ICC/IF=1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 39 kDa
• Target: ALDOB
• UniProt ID: P05062
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: ALDB; ALDO B; ALDO2; ALDOB; ALDOB_HUMAN; Aldolase
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (ALDOB) polyclonal Antibody, Unconjugated (orb101228) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-ALDOB (orb101228) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 39 kD.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human SH-SY5Y cell lysates, Primary: Anti-ALDOB (orb101228) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kDa, Observed band size: 39 kDa.