ALDH5A1 Antibody

• Catalog Number: orb1249024
• Category: Antibodies
• Description: ALDH5A1 Antibody
• Target: ALDH5A1
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-TGKILLHHAANS
• UniProt ID: P51649
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Liver lysates (calculated MW of 58.7kDa according to NP_733936.1). Recommended concentration: 1-3ug/ml. Please note that both this product and EB10435 detects a 50kDa in Mouse and Rat Liver lysates (calculated MW of 56.0kDa according to Mouse NP_766120.1). Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ALDH5A1, aldehyde dehydrogenase 5 family, member A
• Note: For research use only
• NCBI: NP_001071.1, NP_733936.1

orb1249024 (1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249024 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.