ALDH5A1 Antibody

SKU: orb1249024

Description

ALDH5A1 Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 55kDa band observed in Human Liver lysates (calculated MW of 58.7kDa according to NP_733936.1). Recommended concentration: 1-3µg/mL. Please note that both this product and EB10435 detects a 50kDa in Mouse and Rat Liver lysates (calculated MW of 56.0kDa according to Mouse NP_766120.1). Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-TGKILLHHAANS
• Target: ALDH5A1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ALDH5A1, aldehyde dehydrogenase 5 family, member A1, SSADH, SSDH, NAD(+)-dependent succinic semialdehyde dehydrogenase, aldehyde dehydrogenase 5A1, mitochondrial succinate semialdehyde dehydrogenase, succinate-semialdehyde dehydrogenase

orb1249024 (1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249024 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

ALDH5A1

UniProt

P51649

RefSeq (Protein):NP_733936.1, NP_001071.1