ALDH1A1 Antibody

• Catalog Number: orb1268148
• Category: Antibodies
• Description: ALDH1A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-P, WB
• Predicted Reactivity: Monkey, Mouse
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ALDH1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-331 amino acids from the Central region of human ALDH1A1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: ALDH1A1
• UniProt ID: P00352
• NCBI: P00352
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Retinal dehydrogenase 1, RALDH 1, RalDH1, ALDH-E1,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:1000 dilution + human liver lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of ALDH1A1 using rabbit polyclonal ALDH1A1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (Lane 2).

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH1A1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Immunofluorescence analysis of anti-ALDH1A1 Antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H + L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.

Flow cytometric analysis of HepG2 cells using ALDH1A1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.