Alcohol Dehydrogenase/ADH1A Antibody

• Catalog Number: orb381031
• Category: Antibodies
• Description: Alcohol Dehydrogenase/ADH1A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human ADH1A recombinant protein (Position: K213-F375). Human ADH1A shares 87.1% and 82.8% amino acid (aa) sequence identity with mouse and rat ADH1A, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 39859 MW
• UniProt ID: P07327
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Alcohol dehydrogenase 1A;1.1.1.1;Alcohol dehydroge
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-ADH1A antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of ADH1A using anti-ADH1A antibody.Lane 1:human HCCT tissue; 2:human HCCP tissue; 3:rat liver tissue.4:rat kidney tissue.5:mouse liver tissue.6:mouse kidney tissue.

IF analysis of ADH1A using anti-ADH1A antibody. ADH1A was detected in immunocytochemical section of U20S cells.

IHC analysis of ADH1A using anti-ADH1A antibody. ADH1A was detected in a paraffin-embedded section of human liver cancer tissue.

IHC analysis of ADH1A using anti-ADH1A antibody. ADH1A was detected in a paraffin-embedded section of mouse liver tissue.

IHC analysis of ADH1A using anti-ADH1A antibody. ADH1A was detected in a paraffin-embedded section of rat liver tissue.