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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized rh CD166 / ALCAM at 10μg/ml (100μl/well) can bind mouse CD6 Fc Chimera, The EC50 of mouse CD6 Fc Chimera is 0. 1-0. 22μg/ml. |
| Target | ALCAM |
| Tag | His Tag |
| MW | 57.8 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Trp 28 - Ala 526 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | PBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant Human ALCAM Protein [orb2994187]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 82.7 KDa. Observed: 110-125 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Cynomolgus ALCAM Protein [orb2992874]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 56.7 KDa. Observed: 65-115 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Mouse ALCAM Protein [orb2994433]
Unconjugated
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 84.2 KDa. Observed: 100-120 KDa, reducing conditions
50 μg, 500 μg, 1 mg, 10 μgRecombinant Cynomolgus ALCAM Protein [orb2992871]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 82.8 KDa. Observed: 95-133 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μgRecombinant Human ALCAM Protein [orb2992872]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Predicted: 56.8 KDa. Observed: 68-115 KDa, reducing conditions
1 mg, 500 μg, 50 μg, 10 μg

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Human ALCAM, His Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
ALCAM Recombinant Protein (orb1228138)
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