Albumin Rabbit Polyclonal Antibody

• Catalog Number: orb157891
• Category: Antibodies
• Description: Albumin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Human
• Reactivity: Human, Mouse
• CAS Number: 4
• Isotype: IgG
• Immunogen: Mouse serum albumin purified from mouse serum full length
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 68 kDa
• Target: SERPINB14
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Alb 1; ALB; ALBU_MOUSE; Albumin 1; Mouse serum alb
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Mouse serum albumin) Polyclonal Antibody, Unconjugated (orb157891) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse Liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Mouse serum albumin) Polyclonal Antibody, Unconjugated (orb157891) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.

Sample: A549 (Human) Cell Lysate at 30 ug, Primary: Anti-Mouse serum albumin (orb157891) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.

Sample: Lane 1: Mouse Serum, Lane 2: Human Serum, Primary: Anti-Albumin (orb157891) at 1/5000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kDa, Observed band size: 62 kDa.

Sample: Lung (Mouse) Lysate at 40 ug, Spleen (Mouse) Lysate at 40 ug, Primary: Anti-Mouse serum albumin (orb157891) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 68 kD, Observed band size: 68 kD.