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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Target | AKT1 |
| Tag | C-His, C-Twin Strep Tag |
| MW | 59.5 kDa |
| Purity | >92% as determined by reduced SDS-PAGE.Less than 1.0 EU per ug by the LAL method. |
| Protein Sequence | Met 1 - Ala 480 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 20 mM tris, 150 mM NaCl, pH 8.0 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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The purity of rh AKT1/PKB, His.Twin Strep was determined by DTT-reduced (+) SDS-PAGE and staining overnight with Coomassie Blue.
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Protocol Information
AKT1 Recombinant Protein (orb1228196)
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