AKT1 antibody

• Catalog Number: orb422482
• Category: Antibodies
• Description: Goat polyclonal antibody to AKT1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3ug/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 55.7
• Target: AKT1
• Entrez: 207
• Protein Sequence: QDVDQREAPLN
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: AKT1; v-akt murine thymoma viral oncogene homolog
• Note: For research use only
• Application notes: WB: Approx 60kDa band observed in MCF7 cell lysates (calculated MW of 55.7kDa according to NP_005154.2). This molecular weight is routinely observed by other sources. Recommended concentration:0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the microtubules/cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.
• Expiration Date: 12 months from date of receipt.