AKT1 antibody

• Catalog Number: orb345945
• Category: Assays and Kits
• Description: AKT1 antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Anti-AKT1 Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to internal residues of human AKT1 protein. Anti-Mouse IgG HRP secondary antibody was produced by repeated immunizations in rabbit with Mouse IgG whole molecule.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:2,000 - 1: 10,000, FC: User Optimized, IHC: 20 µg/mL, WB: 1: 1000
• Form/Appearance: Liquid and Lyophilized
• Purity: This AKT1 primary and secondary antibody pair has been carefully chosen to give best results. Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected. HRP secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins. This antibody pair is sufficient for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2).
• Conjugation: Unconjugated
• UniProt ID: P31749
• NCBI: NP_001014431.1
• Storage: Primary antibody: the vial contains a relatively low volume of reagent (25 µL). Store vial at -20° C or below prior to opening. To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. Secondary antibody: Store secondary antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Anti-Mouse IgG HRP secondary antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: AKT 1 antibody pair, AKT-1, PKB antibody, PKB gamm
• Note: For research use only
• Application notes: Anti-AKT1 Antibody with the matched secondary are suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This primary monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of His-Tagged Recombinant using AKT1 antibody