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Catalog Number | orb1733246 |
---|---|
Category | Antibodies |
Description | AKT1 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | FC, WB |
Reactivity | Human, Mouse |
Immunogen | between 100 and 150 |
Concentration | 400 µg/ml |
Dilution range | WB: 1:1000 |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | AKT1 |
UniProt ID | P31749 |
Storage | Shelf life: 1 year from date of receipt. Storage: 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | Protein kinase B; RAC; v-akt murine thymoma viral Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human AKT1 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, Jurkat, A-549, and GaMG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (orb1733246) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of mouse AKT1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3 and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (orb1733246) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Detection of human AKT1 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (orb1733246) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of mouse AKT1 (shaded) in CTLL-2 cells by flow cytometry. Antibody: Rabbit anti-AKT1 recombinant monoclonal antibody (orb1733246) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
ELISA, FC, IF, IHC, IP, Multiplex Assay, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-P, WB | |
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, IHC, IP, Multiplex Assay, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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