AKT1 Antibody

• Catalog Number: orb1527295
• Category: Antibodies
• Description: AKT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Immunogen: between 25 and 75
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: AKT1
• UniProt ID: P31749
• NCBI: NP_005154.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: Protein kinase B; RAC; v-akt murine thymoma viral
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human AKT1 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T (T) , MCF-7 (M) , Jurkat (J) and PANC1 (P) cells. Antibody: Affinity purified rabbit anti-AKT1 antibody orb1527295 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human AKT1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-AKT1 (Cat. No. orb1527295) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB