AKT antibody

• Catalog Number: orb1474228
• Category: Antibodies
• Description: Mouse monoclonal antibody to AKT
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Purified recombinant human AKT1 protein fragments expressed in E.coli.AKT1 interacts (via the C-terminus) with CCDC88A (via its C-terminus). Interacts with GRB10; the interaction leads to GRB10 phosphorylation thus promoting YWHAE-binding By similarity. Interacts with AGAP2 (isoform 2/PIKE-A); the interaction occurs in the presence of guanine nucleotides. Interacts with AKTIP. Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CDKN1B; the interaction phosphorylates CDKN1B promoting 14-3-3 binding and cell-cycle progression. Interacts with MAP3K5 and TRAF6. Interacts with BAD, PPP2R5B, STK3 and STK4. Interacts (via PH domain) with SIRT1. Interacts with SRPK2 in a phosphorylation-dependent manner. Interacts with RAF1. Interacts with TRIM13; the interaction ubiquitinates AKT1 leading to its proteasomal degradation. Interacts with TNK2 and CLK2. Interacts (via the C-terminus) with THEM4 (via its C-terminus). Interacts with and phosphorylated by PDPK1.AKT2 interacts (via PH domain) with MTCP1, TCL1A AND TCL1B. Interacts with CLK2, PBH2 and TRAF6. AKT3 interacts (via PH domain) with TCL1A; this enhances AKT3 phosphorylation and activation. Interacts with TRAF6.
• Dilution range: WB (1/500 - 1/1000), IP (1/10 - 1/50)
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Conjugation: Unconjugated
• Target: AKT1; AKT2; AKT3
• Entrez: 11652, 23797, 11651, 208, 207, 10000, 25233, 29414, 24185
• UniProt ID: P31751, Q60823, Q9Y243, Q63484, Q9WUA6, P47196, P31750, P31749, P47197
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: AKT1; PKB; RAC; RAC-alpha serine/threonine-protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of AKT expression in NIH3T3 (A), 293T (B), PC12 (C), K562 (D) whole cell lysates. (Predicted band size: 56 kD; Observed band size: 60 kD)