AKR1C3 Rabbit Polyclonal Antibody

SKU: orb155621

Description

AKR1C3 Rabbit Polyclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Rabbit
• Predicted Reactivity: Bovine, Canine, Equine, Human, Porcine

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human AKR1C3 (161-270/323aa)
• Target: AKR1C3
• Molecular Weight: 36 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DD3; DDX; HA1753; HAKRB; HAKRe; HSD17B5; PGFS; hluPGFS; AK1C3_HUMAN; AKR1C3; 17-beta-hydroxysteroid dehydrogenase type 5 (17-beta-HSD 5); 3-alpha-HSD type II, brain; 3-alpha-hydroxysteroid dehydrogenase type 2 (3-alpha-HSD type 2); Chlordecone reductase homolog HAKRb; Dihydrodiol dehydrogenase 3 (DD-3 | DD3); Dihydrodiol dehydrogenase type I; Prostaglandin F synthase (PGFS); Testosterone 17-beta-dehydrogenase 5; 1.1.1.-; 1.1.1.210; 1.1.1.53; 1.1.1.62; DDH1; KIAA0119;

Paraformaldehyde-fixed, paraffin embedded (Rabbit brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (AKR1C3) Polyclonal Antibody, Unconjugated (orb155621) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Quick Database Links

Gene Symbol

AKR1C3

UniProt

P42330