AKR1B1 Rabbit Polyclonal Antibody

SKU: orb13254

Description

AKR1B1 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Energy Metabolism

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human AKR1B1 (151-250/316aa)
• Target: AKR1B1
• Molecular Weight: 35 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MGC134297; ADR; aldehyde reductase 1; Aldehyde reductase; Aldose reductase; aldr 1; ALDR1; low Km aldose reductase.

Sample: Hela (Human) Cell Lysate at 30 ug, Primary: Anti-AKR1B1 (orb13254) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 34 kD.

Sample: Lane 1: Mouse Adrenal gland tissue lysates, Lane 2: Mouse Testis tissue lysates, Lane 3: Rat Adrenal gland tissue lysates, Lane 4: Rat Testis tissue lysates, Lane 5: Human HepG2 cell lysates, Lane 6: Human A549 cell lysates, Lane 7: Human U87MG cell lysates, Primary: Anti-AKR1B1 (orb13254) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kDa, Observed band size: 35 kDa.

Sample: Testis (Mouse) Lysate at 40 ug, Primary: Anti-AKR1B1 (orb13254) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 34 kD.

Quick Database Links

Gene Symbol

AKR1B1

UniProt

P15121

Fu, Zhong Jie et al. Aldose reductase deficiency reduced vascular changes in neonatal mouse retina in oxygen-induced retinopathy Invest Ophthalmol Vis Sci, 53, 5698-5712 (2012)

PubMed: 22836764