AKR1B1 Antibody

• Catalog Number: orb381037
• Category: Antibodies
• Description: AKR1B1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human AKR1B1 recombinant protein (Position: L228-F316). Human AKR1B1 shares 87.5% amino acid (aa) sequence identity with both mouse and rat AKR1B1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 35853 MW
• UniProt ID: P15121
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Aldose reductase;AR;1.1.1.21;Aldehyde reductase;Al
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-AKR1B1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of AKR1B1 using anti-AKR1B1 antibody.Lane 1:human SiHa cell; 2:rat testis tissue; 3:mouse NIH/3T3 cell.

IHC analysis of AKR1B1 using anti-AKR1B1 antibody.AKR1B1 was detected in paraffin-embedded section of human placenta tissues.

IHC analysis of AKR1B1 using anti-AKR1B1 antibody.AKR1B1 was detected in paraffin-embedded section of rat intestine tissues.

IHC analysis of AKR1B1 using anti-AKR1B1 antibody.AKR1B1 was detected in paraffin-embedded section of rat adrenal gland tissues.