AKR1B1 Antibody / Aldose reductase

• Catalog Number: orb1151720
• Category: Antibodies
• Description: Rabbit monoclonal antibody to AKR1B1
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Recombinant human full-length AKR1B1 protein was used as the immunogen for the recombinant AKR1B1 antibody.
• UniProt ID: P15121
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml
• Application notes: Optimal dilution of the recombinant AKR1B1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: AKR1B1/7009R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the recombinant AKR1B1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This recombinant AKR1B1 antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human brain tissue with recombinant AKR1B1 antibody (clone AKR1B1/7009R) at 2 ug/ml in PBS for 30 min RT. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with recombinant AKR1B1 antibody (clone AKR1B1/7009R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant AKR1B1 antibody (AKR1B1/7009R) as confirmation of integrity and purity.