AIRE antibody

• Catalog Number: orb18719
• Category: Antibodies
• Description: Goat polyclonal antibody to AIRE
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:128000, WB: 1-2 μg/ml, IHC-P: 2.5 μg/ml
• Conjugation: Unconjugated
• MW: 57.7
• Target: AIRE
• Entrez: 326
• Protein Sequence: QSMARPAAPFPS
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti AIRE antibody, anti autoimmune regulator (aut
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration, 2.5ug/ml.WB: Appr. 60kDa in transfected HEK293 lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 0.01-0.03 μg/ml.Experiment Notes: ChIP: A customer has reported this antibody was successful in ChIP (no data).
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Thymus using AIRE antibody

Immunohistochemical staining of Human Thymus using AIRE antibody

Western blot analysis of Human Lymph Node lysate using AIRE antibody

WB analysis of Jurkat cell (A), Human Spleen (B) and Human Smooth Muscle (C) lysate protein using AIRE antibody

Immunofluorescence analysis of U2OS cells using AIRE antibody

Immunofluorescence analysis of HeLa cells using AIRE antibody

Flow cytometric analysis of Jurkat cells using AIRE antibody