AIRE Antibody

• Catalog Number: orb1247825
• Category: Antibodies
• Description: AIRE Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-QSMARPAAPFPS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary testing showed an approx 60kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Smooth Muscle lysate. This product has been successfully used in Western blot by an anonymous customer on HEK293 cell lysate.Immunohistochemistry:In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: AIRE
• UniProt ID: O43918
• NCBI: NP_000374.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: AIRE, autoimmune regulator (automimmune polyendocr
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary testing showed an approx 60kDa band in nuclear lysates of cell line Jurkat and in Human Spleen lysates (calculated MW of 57.7kDa according to NP_000374.1). Recommended concentration: 1-2ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Smooth Muscle lysate. This product has been successfully used in Western blot by an anonymous customer on HEK293 cell lysate.Immunohistochemistry:In paraffin embedded Human Thymic medula shows staining of select nuclei. Recommended concentration: 2.5ug/ml.Immunofluorescence: Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247825 (1 ug/ml) staining of nuclear Jurkat cell (A), (2 ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247825 (2 ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

orb1247825 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nucleoplasm and nuclear membrane staining.

orb1247825 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nucleoplasm and nuclear membrane staining.

orb1247825 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).