AGL Antibody

• Catalog Number: orb1264727
• Category: Antibodies
• Description: AGL Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This AGL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1479-1510 amino acids from the C-terminal region of human AGL.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 175 kDa
• Target: AGL
• UniProt ID: P35573
• NCBI: P35573
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Glycogen debranching enzyme, Glycogen debrancher,
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:8000 dilution + human skeletal muscle lysate Lysates/proteins at 20 ug per lane.

Western blot using anti-AGL antibody at 1:1000 dilution. A total of 20 ug of lysates was loaded for each tissue.

Expression of myc-GS causes wild type but not the ÃCBD mutant of AGL to aggregate around the PAS-stain-positive inclusions. HepG2 cells were transfected with either HA-tagged wild-type AGL (HA-AGL) or HA-AGL ÃCBD. Cells were fixed in formalin and processed for IF using anti-HA (green) and anti-myc (red) antibodies. White arrows indicate colocalization of HA-AGL and myc-GS.

Confocal immunofluorescent analysis of AGL Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).