AGFG1 Antibody

• Catalog Number: orb259588
• Category: Antibodies
• Description: AGFG1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human AGFG1 (5-28aa AKRKQEEKHLKMLRDMTGLPHNRK), identical to the related mouse and rat sequences.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 58260 MW
• UniProt ID: P52594
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Arf-GAP domain and FG repeat-containing protein 1;
• Note: For research use only
• Application notes: WB: The detection limit for AGFG1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-AGFG1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

Western blot analysis using Anti-AGFG1 antibody.Lane 1:Rat Testis Tissue;2:Rat Liver Tissue;3:Human Placenta Tissue;4:MCF-7 Cell;5:HELA Cell.

IF analysis of AGFG1 using anti-AGFG1 antibody. AGFG1 was detected in immunocytochemical section of U20S cells.