AGER Antibody

• Catalog Number: orb1671723
• Category: Antibodies
• Description: AGER Antibody
• Clonality: Recombinant
• Clone Number: 2A11
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: Human RAGE protein.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: AGER
• UniProt ID: Q15109
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: AGER, advanced glycosylation end product-specific
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-RAGE antibody 2A11. A549 cells were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - shaded line) or the r version of 2A11 (orb1671723 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of A549 cells using anti-RAGE antibody (2A11). Immunofluorescence analysis of paraformaldehyde fixed A549 cells stained with the chimeric r version of 2A11 (orb1671723) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671723- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

Western Blot using anti-RAGE antibody 2A11. Human lung lysate (35 ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric r version of 2A11 (orb1671723) at 0.03 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.