AFF1 Antibody

• Catalog Number: orb1254354
• Category: Antibodies
• Description: AFF1 Antibody
• Target: AFF1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Liquid
• Purification: Affinity purification
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1124-1218 of human AFF1 (NP_001160165.1).
• UniProt ID: P51825
• MW: Observed: 135kDa
• Tested applications: IF, IHC, WB
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AFF1, AF4/FMR2 family, member 1, AF-4, AF4, AF4/FM
• Research Area: Cancer Biology, Epigenetics & Chromatin
• Note: For research use only

Western blot analysis of extracts of various cell lines, using AFF1 antibody (orb1254354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Immunofluorescence analysis of U2OS cells using AFF1 antibody (orb1254354).