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AFF4 Rabbit Polyclonal Antibody

SKU: orb3107003

Description

The AFF4 Rabbit Polyclonal Antibody (Carrier-free) is a Carrier-free antibody raised in rabbit. It is immunogen affinity purified and suitable for use in FC, IHC, IP, and WB applications. This antibody targets the AF4/FMR2 family member 4 in Human, Mouse, and Rat samples.

Research Area

Cell Biology, Epigenetics & Chromatin

Images & Validation

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Tested ApplicationsFC, IHC, IP, WB
Dilution RangeWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Immunoprecipitation, 0.5-2 μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
ReactivityHuman, Mouse, Rat
Application Notes
For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.

Related Conjugates & Formulations

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Key Properties

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Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the N-terminus of human AFF4, identical to the related mouse sequence.
TargetAF4/FMR2 family member 4
Molecular Weight150 kDa
PurificationImmunogen affinity purified.
ConjugationCarrier-free

Storage & Handling

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StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

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AF4/FMR2 family member 4; AF4/FMR2 family; member 4; AF5Q31; AFF4; MCEF; Protein AF 5q31

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Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at support@biorbyt.com.

UniProt Details

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No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IP
Immunoprecipitation
View Protocol

Available Sizes

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100 μg
$ 470.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-4 weeks
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