ADRB3 Rabbit Polyclonal Antibody

• Catalog Number: orb221343
• Category: Antibodies
• Description: ADRB3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Equine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADRB3 (51-150/408aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 44 kDa
• Target: ADRB3
• UniProt ID: P13945
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: beta 3 Adrenergic Receptor; beta3-adrenergic recep
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Sreenath Kundimi, Gopichand Chinta, Krishnaraju Venkata Alluri, Trimurtulu Golakoti, Sudipta Veeramachaneni, Guru Ramanathan, Krishanu Sengupta A Synergistic Botanical Composition Increases Resting Energy Expenditure and Reduces Adiposity in High-Fat Diet-Fed Rats J Am Nutr Assoc, 1~10 (2023)

PubMed: 38015050

Applications

IHC

Reactivity

Rat

Sample: Lane 1: Mouse Urinary bladder tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Stomach tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Human HeLa cell lysates, Lane 6: Human U-2 OS cell lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human SH-SY5Y cell lysates, Primary: Anti-ADRB3 (orb221343) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 48 kDa.

Sample: mesentery (mouse) Lysate at 40 ug, lymph node (Mouse) Lysate at 40 ug, Primary: Anti-ADRB3/beta 3 Adrenergic Receptor (orb221343) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 44 kD.

Tissue/Cell: Human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated (orb221343) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Rat adrenal gland, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated (orb221343) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.