ADRB3 Rabbit Polyclonal Antibody

SKU: orb221343

Description

ADRB3 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Equine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADRB3 (51-150/408aa)
• Target: ADRB3
• Molecular Weight: 48 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

BETA3AR; Adrb-3; beta 3-AR; ADRB; ADRB3_HUMAN; ADRB3; Beta-3 adrenoreceptor (Beta-3 adrenoceptor); ADRB3R; B3AR; ADRB3_MOUSE; B3bar; ADRB3_RAT; adrenoceptor beta 3; adrenergic, beta-3-, receptor

Sample: Lane 1: Mouse Urinary bladder tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Mouse Stomach tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Human HeLa cell lysates, Lane 6: Human U-2 OS cell lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human SH-SY5Y cell lysates, Primary: Anti-ADRB3 (orb221343) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kDa, Observed band size: 48 kDa.

Sample: mesentery (mouse) Lysate at 40 ug, lymph node (Mouse) Lysate at 40 ug, Primary: Anti-ADRB3/beta 3 Adrenergic Receptor (orb221343) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 44 kD, Observed band size: 44 kD.

Tissue/Cell: Human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated (orb221343) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: Rat adrenal gland, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated (orb221343) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

ADRB3

UniProt

P13945

Sreenath Kundimi, Gopichand Chinta, Krishnaraju Venkata Alluri, Trimurtulu Golakoti, Sudipta Veeramachaneni, Guru Ramanathan, Krishanu Sengupta A Synergistic Botanical Composition Increases Resting Energy Expenditure and Reduces Adiposity in High-Fat Diet-Fed Rats J Am Nutr Assoc, 1~10 (2023)

PubMed: 38015050

Applications

IHC

Reactivity

Rat