ADRB2 Rabbit Polyclonal Antibody

• Catalog Number: orb10056
• Category: Antibodies
• Description: ADRB2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADRB2 (201-300/418aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 46 kDa
• Target: ADRB2
• UniProt ID: P07550
• RRID: AB_10747011
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: beta 2-adrenergic receptor; beta 2 Adrenergic Rece
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ADRB2) Polyclonal Antibody, Unconjugated (orb10056) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Positive control: (mo) Splenocytes (2% Paraformaldehyde-fixed), Isotype Control Antibody, Antibody: Rabbit IgG, Dilution: 1 µg in 100 µl 1 X PBS containing 0.5% BSA, Secondary Antibody, Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody, catalog number: orb10056, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Mouse Stomach tissue lysates, Lane 3: Mouse Cerebrum tissue lysates, Lane 4: Rat Heart tissue lysates, Lane 5: Rat Stomach tissue lysates, Lane 6: Rat Cerebrum tissue lysates, Lane 7: Human A431 cell lysates, Primary: Anti-TBX1 (orb10056) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 46 kDa, Observed band size: 50 kDa.