ADRA2C Rabbit Polyclonal Antibody

• Catalog Number: orb155600
• Category: Antibodies
• Description: ADRA2C Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human ADRA2C/alpha 2C Adrenergic Receptor (21-120/462aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 49 kDa
• Target: ADRA2C
• UniProt ID: P18825
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha 2C Adrenergic Receptor; Adra 2c; Adra 2c; AD
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): A431 (fixed with 70% ice-cold methanol overnight at 4°C). Primary Antibody (green line): Rabbit Anti-ADRA2C/PE-CY5 Conjugated antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-PE-CY5.

Blank control (blue): U937 (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-ADRA2C antibody (orb155600), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human SH-SY5Y cell lysate, Lane 4: Human 293T cell lysates, Lane 5: Human A549 cell lysates, Primary: Anti-ADRA2C (orb155600) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 49 kDa, Observed band size: 58 kDa.