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    ADORA2A antibody

    Catalog Number: orb213530

    DispatchUsually dispatched within 5-10 working days
    $ 337.00
    Catalog Numberorb213530
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to ADORA2A
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Primate, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the center region of human Adenosine A2a Receptor. The exact sequence is proprietary.
    Dilution rangeWB: 1-500-1-1000, IF/ICC: 1-100-1-500
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetADORA2A
    Entrez25369, 11540, 135
    UniProt IDQ60613, P29274, P30543
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti Adenosine Receptor A2a antibody, anti Adenosi
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.
    ADORA2A antibody

    Western blot analysis of Adenosine A2a Receptor expression in HEK293T (A), Hela (B), HepG2 (C), mouse brain (D), rat liver (E), mouse kidney (F) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 70; 45 kD)

    ADORA2A antibody

    Immunohistochemical analysis of Adenosine A2a Receptor staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    ADORA2A antibody

    Immunofluorescent analysis of Adenosine A2a Receptor staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

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