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    Adenosine A2a Receptor antibody

    Catalog Number: orb338920

    DispatchUsually dispatched within 5-10 working days
    $ 413.00
    Catalog Numberorb338920
    CategoryAntibodies
    DescriptionRabbit polyclonal antibody to ADORA2A
    Species/HostRabbit
    ClonalityPolyclonal
    Tested applicationsIF, IH, WB
    ReactivityHuman, Mouse, Rat
    ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Adenosine A2a Receptor. The exact sequence is proprietary.
    Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
    Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    ConjugationUnconjugated
    TargetADORA2A
    Entrez25369, 11540, 135
    UniProt IDQ60613, P29274, P30543
    SourceRabbit
    StorageShipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
    Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
    Alternative namesanti ADORA2 antibody, anti Adenosine receptor A2a
    Read more...
    NoteFor research use only
    Expiration Date12 months from date of receipt.

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    Applications

    IHC

    Reactivity

    Rat
    Adenosine A2a Receptor antibody

    Western blot analysis of Adenosine A2a Receptor expression in Hela (A), HepG2 (B), NIH3T3 (C), H9C2 (D) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 45 kD)

    Adenosine A2a Receptor antibody

    Immunohistochemical analysis of Adenosine A2a Receptor staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    Adenosine A2a Receptor antibody

    Immunofluorescent analysis of Adenosine A2a Receptor staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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      ELISA,  IF,  IHC

      Human

      Rabbit

      Polyclonal

      Unconjugated

      100 μg, 50 μg
    • ADORA2A antibody [orb213530]

      IF,  IH,  WB

      Human, Mouse, Primate, Rat

      Rabbit

      Polyclonal

      Unconjugated

      200 μl, 100 μl, 30 μl
    • ADORA2A antibody [orb767652]

      ELISA,  IF,  IHC-P,  WB

      Human

      Rabbit

      Polyclonal

      Unconjugated

      50ul, 100ul
    • ADORA2A antibody [orb555909]

      WB

      Human, Rat

      Rabbit

      Polyclonal

      Unconjugated

      100 μl
    • ADORA2A antibody [orb306397]

      FC,  IHC-P,  WB

      Human

      Rabbit

      Polyclonal

      Unconjugated

      80 μl
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